產品英文名稱：CHO-CM HCP ELISA kit
產品中文名稱：CHO-CM HCP ELISA 試劑盒

CHO-CM HCP ELISA kit 的應用
本試劑盒是用于確定在CHO細胞重組表達產品中CHO宿主細胞蛋白（HCPS）的存在。本試劑盒適合使用的抗體通常發(fā)現(xiàn)在無蛋白的條件培養(yǎng)介質相生成。該試劑盒是用于研究和制造使用，不用于人類或動物的診斷應用。

：，客服

CHO-CM HCP ELISA 試劑盒的英文描述
This assay is intended for use on pharmaceutical products secreted into protein-free CHO growth media. The antibody is raised against, affinity purified, and calibrated with HCPs typically found in CHO protein-free conditioned media. Special procedures were utilized in the generation of these antibodies to insure that low molecular weight and less immunogenic contaminants as well as high molecular weight components would be represented. Our assay validation has determined that the LOD for this kit is <200pg/mL.

CHO-CM HCP ELISA kit 的使用步驟規(guī)則
The CHO HCP assay is a sequential, two-site immunoenzymetric assay also termed ELISA. Samples potentially containing CHO proteins are reacted in microtiter strips coated with an affinity purified capture antibody. After a wash step, a horseradish peroxidase enzyme labeled anti-CHO antibody is reacted sequentially forming a sandwich complex of solid phase antibody-CHO protein-enzyme labeled antibody. The microtiter strips are then washed to remove any unbound reactants. The substrate tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of CHO proteins present.

CHO-CM HCP ELISA kit 的保存和穩(wěn)定性
* All reagents should be stored at 2C to 8C for stability until the expiration date printed.
*Reconstituted wash solution is stable until the expiration date of the kit.

CHO-CM HCP ELISA kit 的使用注意事項
*只用于研究或制造。
*停止反應試劑是0.5M H2SO4。避免接觸眼睛，皮膚，和服裝。在用本試劑盒的試劑濃度，其他沒有被認為是有害的。
*本試劑盒只能由合格的技術人員使用。
 

一切干細胞實驗耗材優(yōu)惠盡在華雅思創(chuàng)

2015年統(tǒng)統(tǒng)*，--一切活動盡在上海華雅思創(chuàng)！

：，客服

StemRD   MGro-500  MSC medium,chemically-defined,間充質干細胞培養(yǎng)基 500mL/瓶

LONZA   04-418Q    X-VIVO 15 無血清培養(yǎng)基 1L/瓶

Life Technology   087-0112DK    AIM-V® CTS™ 無血清細胞培養(yǎng)基（細胞治療級） 1000mL/瓶

Pall   15950-017  Ultroser Serum Substitute 血清替代品 20mL/瓶

LONZA   12-725F   UltraCULTURE Serum-free Medium無血清培養(yǎng)基 500mL/瓶

BD 762165   PAXgene Blood RNA Tube RNA樣本收集管 100管/盒

LONZA 50150   SeaKem® Gold Agarose 金瓊脂糖粉 125g/瓶

LONZA CC-3162   EGM-2 BulletKit( CC-3156 & CC-4176 )套裝 Kit/套

Nunc 159910   175cm2易用培養(yǎng)瓶,PS,已滅菌,瓶蓋:過濾蓋 30個/箱

LONZA LT07-318  MycoAlert® Mycoplasma Detection Kit支原體檢測試劑盒 100Tests/盒

LONZA   LT07-418 MycoAlert® Mycoplasma Detection Kit支原體檢測試劑盒 50Tests/盒

Lonza   50-650U   Kinetic-QCL 192 Test Kit內毒素檢測試劑盒   192T/盒

Corning   431080    175cm⒉細胞培養(yǎng)瓶，Angled頸，透氣蓋 50個/箱

Corning   8388  125mL儲液瓶 Storage Bottles,PS,45mm瓶頸，帶蓋，滅菌，獨立包裝 24個/箱

Corning   430659    2ml凍存管, 聚丙烯，外旋蓋, 可自立圓底 500個/箱

Corning   3290     75cm2 CellBIND Surface培養(yǎng)瓶，已滅菌，透氣蓋 100個/箱

Corning   3292     175cm2 CellBIND Surface培養(yǎng)瓶，已滅菌，透氣蓋 50個/箱

BD-Falcon   352360   Cell Strainer, 100µm, Yellow 100um細胞濾網 50個/箱

BD Falcon 352070 50mL錐形離心管，高透明度聚丙烯,袋裝 500支/箱

BD Falcon 352096 15mL錐形離心管，高透明度聚丙烯,袋裝 500支/箱

BD BioCoat 354234   Matrigel Basement Membrane Matrix(標準型) 10mL/瓶

Cygnus   F550   CHO HCP ELISA Kit,3G宿主蛋白檢測試劑盒 96T/盒

Cygnus   F410   E.coli HCP ELISA Kit 大腸桿菌 96T/盒

Nalgene  5100-0001 Cryo 1℃ Freezing Container梯度降溫凍存盒 1個/箱

Nalgene    5026-0909   CryoBoxes 凍存盒 9X9 陣列，管瓶1.2/2.0mL   24個/箱

Nalgene   2250-0130 50L 可高溫高壓操作的細口大瓶（帶手柄）   1個/箱

Nalgene   2319-0050    20L 可高溫高壓滅菌的細口大瓶（帶放水口）    1個/箱

Hyclone  SH30084.03 FBS 優(yōu)等澳洲胎牛血清 500mL/瓶

PeproTech   100-18B-500   Recombinant Human FGF-basic (154 a.a.)因子 500ug/支

Parafilm PM-996   Parafilm® M Laboratory Film 封口膜 12卷/箱