簡(jiǎn)要描述:英文名稱(chēng): Activin A,human產(chǎn)品應(yīng)用: 胚胎發(fā)育、細(xì)胞生長(zhǎng)分化中國(guó)俗稱(chēng): 激活素A 注冊(cè)品牌: StemRD 生產(chǎn)廠家: 美國(guó)StemRD公司訂購(gòu)貨號(hào): ACT-025規(guī)格型號(hào): 25?g保存溫度: ?-80℃產(chǎn)品種類(lèi): 生長(zhǎng)分化因子到貨時(shí)間: 1~5天
產(chǎn)品目錄
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產(chǎn)品英文名稱(chēng):Activin A,human
產(chǎn)品中文名稱(chēng): 人激活素A
訂購(gòu):
:gnana@hystemcell.com
Activin A,human產(chǎn)品介紹:
激活素(activins) Activin A是轉(zhuǎn)化生長(zhǎng)因子(TGF)β家族的一種分泌蛋白,可控制胚胎軸發(fā)育為有功能的前腸源性組織,調(diào)解多種細(xì)胞的生長(zhǎng)和分化。多種研究表明,其能誘導(dǎo)胰腺β細(xì) 胞的分化。尤其與其他因子的聯(lián)合作用后能發(fā)揮更顯著的誘導(dǎo)作用。如activin A聯(lián)合全反式維甲酸可以更有效地誘導(dǎo)鼠ESC為產(chǎn)胰島素細(xì)胞,而activin與BTC共同作用可以轉(zhuǎn)化新生大鼠導(dǎo)管細(xì)胞為產(chǎn)胰島素細(xì)胞。卵泡抑素 (follistatin)和表皮生長(zhǎng)因子相關(guān)肽(Cripto)是ac2tivins的天然拮抗劑,有研究表明了胰島分化階段導(dǎo)管上皮細(xì)胞里的 activins及其抑制因子follistatin和Cripto都有所增加,卵泡抑素抑制activin的同時(shí),能顯著促進(jìn)胰腺導(dǎo)管細(xì)胞新生而減少β 細(xì)胞的分化,故選擇抑制activin信號(hào)途徑可運(yùn)用于擴(kuò)增前體細(xì)胞,而激活activin有利于β細(xì)胞的分化。這些結(jié)果表說(shuō)明,胰腺生長(zhǎng)和分化之間的平 衡可通過(guò)activin信號(hào)途徑的精確調(diào)節(jié)來(lái)實(shí)現(xiàn)。同時(shí)它還被廣泛用于干細(xì)胞研究,用來(lái)誘導(dǎo)分化的胚胎干細(xì)胞形成內(nèi)胚層。
Activin A,human 產(chǎn)品特性:
? Activin A,human內(nèi)毒素含量小于0.1EU /μg,活性經(jīng)過(guò)人源細(xì)胞鑒定,純度高
? 生物活性在人源293細(xì)胞經(jīng)過(guò)SMAD實(shí)驗(yàn)鑒定The EC50為 0.5–2.0 ng/ml
? Activin A,human產(chǎn)品經(jīng)過(guò)SDS-PAGE 和HPLC雙層鑒定純度大于95%
? 只限于科學(xué)研究,非人體使用
Activin A,human 產(chǎn)品英文簡(jiǎn)述:
Activin A is a member of the TGFβ superfamily. It is a 26 kDa disulfidelinked homodimer of two βA chains, each containing 116 amino acid residues. Activin A is widely used in stem cell research. It has been used to induce the differentiation of embryonic stem cells to the definitive endoderm. The biological activities of Activin A can be neutralized by diffusible TGF-β antagonists, Follistatin (also available from StemRD). StemRD's Activin A is made in human 293 cells, which renders human-specific post-translational modifications. We purified this product through a series of chromatography including HPLC to near homogeneity.
? Purity: Greater than 95% by SDS-PAGE and HPLC analysis
? Biological Activity: The activity was determined by using a SMAD reporter gene assay in human 293 cells. The EC50 ranges from 0.5 - 2.0 ng/ml
? For Research Use Only. Not for Use in Humans
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StemRD MGro-500 MSC medium,chemically-defined,間充質(zhì)干細(xì)胞培養(yǎng)基 500mL/瓶
LONZA 04-418Q X-VIVO 15 無(wú)血清培養(yǎng)基 1L/瓶
Life Technology 087-0112DK AIM-V? CTS? 無(wú)血清細(xì)胞培養(yǎng)基(細(xì)胞治療級(jí)) 1000mL/瓶
Pall 15950-017 Ultroser Serum Substitute 血清替代品 20mL/瓶
LONZA 12-725F UltraCULTURE Serum-free Medium無(wú)血清培養(yǎng)基 500mL/瓶
BD 762165 PAXgene Blood RNA Tube RNA樣本收集管 100管/盒
LONZA 50150 SeaKem? Gold Agarose 金瓊脂糖粉 125g/瓶
LONZA CC-3162 EGM-2 BulletKit( CC-3156 & CC-4176 )套裝 Kit/套
Nunc 159910 175cm2易用培養(yǎng)瓶,PS,已滅菌,瓶蓋:過(guò)濾蓋 30個(gè)/箱
LONZA LT07-318 MycoAlert? Mycoplasma Detection Kit支原體檢測(cè)試劑盒 100Tests/盒
LONZA LT07-418 MycoAlert? Mycoplasma Detection Kit支原體檢測(cè)試劑盒 50Tests/盒
Lonza 50-650U Kinetic-QCL 192 Test Kit內(nèi)毒素檢測(cè)試劑盒 192T/盒
Corning 431080 175cm⒉細(xì)胞培養(yǎng)瓶,Angled頸,透氣蓋 50個(gè)/箱
Corning 8388 125mL儲(chǔ)液瓶 Storage Bottles,PS,45mm瓶頸,帶蓋,滅菌,獨(dú)立包裝 24個(gè)/箱
Corning 430659 2ml凍存管, 聚丙烯,外旋蓋, 可自立圓底 500個(gè)/箱
Corning 3290 75cm2 CellBIND Surface培養(yǎng)瓶,已滅菌,透氣蓋 100個(gè)/箱
Corning 3292 175cm2 CellBIND Surface培養(yǎng)瓶,已滅菌,透氣蓋 50個(gè)/箱
BD-Falcon 352360 Cell Strainer, 100μm, Yellow 100um細(xì)胞濾網(wǎng) 50個(gè)/箱
BD Falcon 352070 50mL錐形離心管,高透明度聚丙烯,袋裝 500支/箱
BD Falcon 352096 15mL錐形離心管,高透明度聚丙烯,袋裝 500支/箱
BD BioCoat 354234 Matrigel Basement Membrane Matrix(標(biāo)準(zhǔn)型) 10mL/瓶
Cygnus F550 CHO HCP ELISA Kit,3G宿主蛋白檢測(cè)試劑盒 96T/盒
Cygnus F410 E.coli HCP ELISA Kit 大腸桿菌 96T/盒
Nalgene 5100-0001 Cryo 1℃ Freezing Container梯度降溫凍存盒 1個(gè)/箱
Nalgene 5026-0909 CryoBoxes 凍存盒 9X9 陣列,管瓶1.2/2.0mL 24個(gè)/箱
Nalgene 2250-0130 50L 可高溫高壓操作的細(xì)口大瓶(帶手柄) 1個(gè)/箱
Nalgene 2319-0050 20L 可高溫高壓滅菌的細(xì)口大瓶(帶放水口) 1個(gè)/箱
Hyclone SH30084.03 FBS 優(yōu)等澳洲胎牛血清 500mL/瓶
PeproTech 100-18B-500 Recombinant Human FGF-basic (154 a.a.)因子 500ug/支
Parafilm PM-996 Parafilm? M Laboratory Film 封口膜 12卷/箱